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Deficiency of expansion in glucose of S. cerevisiae tps1 mutants has been attributed to loss of inhibition by T6P of hexokinase two the glucose phosphorylating enzyme expressed during expansion in this sugar. Because T6P inhibition of Y. lipolytica hexokinase is the highest documented (Ki 3.five mM) we researched the impact of the disruption of TPS1 in this yeast. Makes an attempt to disrupt YlTPS
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The protein putatively encoded by this ORF is highly comparable to the S. cerevisiae Tfc1, 1 of the two DNAbinding subunits of the yeast transcription element TFIIIC. Element of the TFC1 promoter very likely overlaps with the coding sequence of YlTPS1. The relative place of these ORFs in Y. lipolytica is various from that discovered in other Hemiascomycetes in which TPS1 seems in a synteny block t
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