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Ed at least from 3 individual batches of mature mast cells. Each batch of mast cells was grown from femoral bone marrows of two mice.Reagents and antibodiesAgNPs with a diameter of 20 nm were procured from NanoComposix (San Diego, CA). For most studies, we used a concentration of 25 g/ml based on our previous findings and others [12, 21]. AgNPs are suspended in citrate at a concentration of 1 mg/m